Thiocyanate, a plausible physiological electron donor of gastric peroxidase
نویسندگان
چکیده
منابع مشابه
Thiocyanate, a plausible physiological electron donor of gastric peroxidase.
Gastric peroxidase (GPO) was purified to apparent homogeneity to characterize its major physiological electron donor. The enzyme (RZ = 0.7), with a subunit molecular mass of 50 kDa, is a glycoprotein, with a relative abundance of aspartic and glutamic acid over arginine and lysine. It has a Soret maximum at 412 nm, which is shifted to 426 nm by H2O2 due to formation of compound II. Although the...
متن کاملCharacterization of sheep lacrimal-gland peroxidase and its major physiological electron donor.
A soluble sheep lacrimal-gland peroxidase was purified to apparent homogeneity. It had a native molecular mass of 75 kDa with a subunit molecular mass of 82 kDa and an isoelectric point of 6.5. Western blotting showed that it shares some of the enzyme antigenic determinants in common with other soluble peroxidases. The enzyme exhibits a Soret peak at 410 nm which is shifted to 431 nm by 5 equiv...
متن کاملInvestigation of electron and hydrogenic-donor states confined in a permeable spherical box using B-splines
Effects of quantum size and potential shape on the spectra of an electron and a hydrogenic-donor at the center of a permeable spherical cavity have been calculated, using linear variational method. B-splines have been used as basis functions. By extensive convergence tests and comparing with other results given in the literature, the validity and efficiency of the method were confirmed.
متن کاملLow catalytic turnover of horseradish peroxidase in thiocyanate oxidation. Evidence for concurrent inactivation by cyanide generated through one-electron oxidation of thiocyanate.
The catalytic turnover of horseradish peroxidase (HRP) to oxidize SCN- is a hundredfold lower than that of lactoperoxidase (LPO) at optimum pH. While studying the mechanism, HRP was found to be reversibly inactivated following pseudo-first order kinetics with a second order rate constant of 400 M-1 min-1 when incubated with SCN- and H2O2. The slow rate of SCN- oxidation is increased severalfold...
متن کاملPlasma and salivary thiocyanate in gastric cancer.
Previous studies (Jakobi, Jakobi, and Cavalcanti, 1961) have suggested that the concentration of thiocyanate in the saliva of patients suffering from oesophageal or gastric cancer is unusually low compared with that in normal subjects or patients with peptic ulcer. If this were confirmed the measurement of salivary thiocyanates might provide a screening test for gastric cancer. Salivary thiocya...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1995
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj3050059